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Development and Application of a Most-Probable-Number–PCR Assay To Quantify Flagellate Populations in Soil Samples

This paper reports on the first successful molecular detection and quantification of soil protozoa. Quantification of heterotrophic flagellates and naked amoebae in soil has traditionally relied on dilution culturing techniques, followed by most-probable-number (MPN) calculations. Such methods are b...

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Detalhes bibliográficos
Main Authors: Fredslund, Line, Ekelund, Flemming, Jacobsen, Carsten Suhr, Johnsen, Kaare
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2001
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC92777/
https://ncbi.nlm.nih.gov/pubmed/11282613
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.67.4.1613-1618.2001
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