Evaluation of Various Campylobacter-Specific Quantitative PCR (qPCR) Assays for Detection and Enumeration of Campylobacteraceae in Irrigation Water and Wastewater via a Miniaturized Most-Probable-Number–qPCR Assay

Campylobacter spp. are the leading cause of bacterial gastroenteritis worldwide, and water is increasingly seen as a risk factor in transmission. Here we describe a most-probable-number (MPN)–quantitative PCR (qPCR) assay in which water samples are centrifuged and aliquoted into microtiter plates an...

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Veröffentlicht in:Appl Environ Microbiol
Hauptverfasser: Banting, Graham S., Braithwaite, Shannon, Scott, Candis, Kim, Jinyong, Jeon, Byeonghwa, Ashbolt, Nicholas, Ruecker, Norma, Tymensen, Lisa, Charest, Jollin, Pintar, Katarina, Checkley, Sylvia, Neumann, Norman F.
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology 2016
Schlagworte:
Public and Environmental Health Microbiology
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4984289/
https://ncbi.nlm.nih.gov/pubmed/27235434
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00077-16
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