Portable System for Microbial Sample Preparation and Oligonucleotide Microarray Analysis

We have developed a three-component system for microbial identification that consists of (i) a universal syringe-operated silica minicolumn for successive DNA and RNA isolation, fractionation, fragmentation, fluorescent labeling, and removal of excess free label and short oligonucleotides; (ii) micr...

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Main Authors: Bavykin, Sergei G., Akowski, James P., Zakhariev, Vladimir M., Barsky, Viktor E., Perov, Alexander N., Mirzabekov, Andrei D.
Format: Artigo
Jezik:Inglês
Izdano: American Society for Microbiology 2001
Teme:
Methods
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC92667/
https://ncbi.nlm.nih.gov/pubmed/11157263
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.67.2.922-928.2001
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