Microvariation Artifacts Introduced by PCR and Cloning of Closely Related 16S rRNA Gene Sequences
A defined template mixture of seven closely related 16S-rDNA clones was used in a PCR-cloning experiment to assess and track sources of artifactual sequence variation in 16S rDNA clone libraries. At least 14% of the recovered clones contained aberrations. Artifact sources were polymerase errors, a m...
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| Vydáno v: | Appl Environ Microbiol |
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| Hlavní autoři: | , , , , , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
American Society for Microbiology (ASM)
2001
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC92603/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11133483/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.67.1.469-472.2001 |
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