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Microvariation Artifacts Introduced by PCR and Cloning of Closely Related 16S rRNA Gene Sequences
A defined template mixture of seven closely related 16S-rDNA clones was used in a PCR-cloning experiment to assess and track sources of artifactual sequence variation in 16S rDNA clone libraries. At least 14% of the recovered clones contained aberrations. Artifact sources were polymerase errors, a m...
Gorde:
| Egile Nagusiak: | , , , , , |
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| Formatua: | Artigo |
| Hizkuntza: | Inglês |
| Argitaratua: |
American Society for Microbiology
2001
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| Gaiak: | |
| Sarrera elektronikoa: | https://ncbi.nlm.nih.gov/pmc/articles/PMC92603/ https://ncbi.nlm.nih.gov/pubmed/11133483 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.67.1.469-472.2001 |
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