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Microvariation Artifacts Introduced by PCR and Cloning of Closely Related 16S rRNA Gene Sequences

A defined template mixture of seven closely related 16S-rDNA clones was used in a PCR-cloning experiment to assess and track sources of artifactual sequence variation in 16S rDNA clone libraries. At least 14% of the recovered clones contained aberrations. Artifact sources were polymerase errors, a m...

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Udgivet i:Appl Environ Microbiol
Principais autores: Speksnijder, Arjen G. C. L., Kowalchuk, George A., De Jong, Sander, Kline, Elizabeth, Stephen, John R., Laanbroek, Hendrikus J.
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 2001
Fag:
Microbial Ecology
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC92603/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11133483/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.67.1.469-472.2001
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