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Advances in Development of a Genetic System for Thermoanaerobacterium spp.: Expression of Genes Encoding Hydrolytic Enzymes, Development of a Second Shuttle Vector, and Integration of Genes into the Chromosome

Despite recent success in transforming various thermophilic gram-type-positive anaerobes with plasmid DNA, use of shuttle vectors for the expression of genes other than antibiotic resistance markers has not previously been described. We constructed new vectors in order to express heterologous hydrol...

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Vydáno v:Appl Environ Microbiol
Hlavní autoři: Mai, Volker, Wiegel, Juergen
Médium: Artigo
Jazyk:Inglês
Vydáno: American Society for Microbiology (ASM) 2000
Témata:
On-line přístup:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC92385/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11055929/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.66.11.4817-4821.2000
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