Advances in Development of a Genetic System for Thermoanaerobacterium spp.: Expression of Genes Encoding Hydrolytic Enzymes, Development of a Second Shuttle Vector, and Integration of Genes into the Chromosome

Despite recent success in transforming various thermophilic gram-type-positive anaerobes with plasmid DNA, use of shuttle vectors for the expression of genes other than antibiotic resistance markers has not previously been described. We constructed new vectors in order to express heterologous hydrol...

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Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	Mai, Volker, Wiegel, Juergen
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 2000
Assuntos:	Physiology and Biotechnology
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC92385/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11055929/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.66.11.4817-4821.2000
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