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Advances in Development of a Genetic System for Thermoanaerobacterium spp.: Expression of Genes Encoding Hydrolytic Enzymes, Development of a Second Shuttle Vector, and Integration of Genes into the Chromosome

Despite recent success in transforming various thermophilic gram-type-positive anaerobes with plasmid DNA, use of shuttle vectors for the expression of genes other than antibiotic resistance markers has not previously been described. We constructed new vectors in order to express heterologous hydrol...

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Pubblicato in:Appl Environ Microbiol
Autori principali: Mai, Volker, Wiegel, Juergen
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Microbiology (ASM) 2000
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Accesso online:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC92385/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11055929/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.66.11.4817-4821.2000
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