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The Complete Amino Acid Substitutions at Position 131 That Are Positively Involved in Cold Adaptation of Subtilisin BPN′

To ascertain whether position 131 of a mesophilic protease, subtilisin BPN′, is a potential critical site for cold adaptation as screened by evolutionary engineering (S. Taguchi, A. Ozaki, and H. Momose, Appl. Environ. Microbiol. 64:492–495, 1998), a full set of subtilisin BPN′ mutants with mutation...

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Udgivet i:Appl Environ Microbiol
Principais autores: Taguchi, Seiichi, Komada, Shingo, Momose, Haruo
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 2000
Fag:
Enzymology and Protein Engineering
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC92001/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10742220/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.66.4.1410-1415.2000
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