Alteration of Substrate Specificity and Optimum pH of Sarcosine Oxidase by Random and Site-Directed Mutagenesis

The sarcosine oxidase gene was randomly mutated, and F103V, which showed altered specificity, was screened with the plate assay. Five different mutants at position 103 were also constructed. Each mutant exhibited a rather striking change in substrate specificity, except for F103W. The optimum pH was...

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Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	Nishiya, Yoshiaki, Imanaka, Tadayuki
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1994
Assuntos:	Enzymology and Protein Engineering
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC201966/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16349451/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.60.11.4213-4215.1994
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