Cloning, Characterization, Controlled Overexpression, and Inactivation of the Major Tributyrin Esterase Gene of Lactococcus lactis

The gene encoding the major intracellular tributyrin esterase of Lactococcus lactis was cloned using degenerate DNA probes based on 19 known N-terminal amino acid residues of the purified enzyme. The gene, named estA, was sequenced and found to encode a protein of 258 amino acid residues. The transc...

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Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	Fernández, Leonides, Beerthuyzen, Marke M., Brown, Julie, Siezen, Roland J., Coolbear, Tim, Holland, Ross, Kuipers, Oscar P.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 2000
Assuntos:	Genetics and Molecular Biology
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC91993/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10742212/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.66.4.1360-1368.2000
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