Cloning, Characterization, Controlled Overexpression, and Inactivation of the Major Tributyrin Esterase Gene of Lactococcus lactis

The gene encoding the major intracellular tributyrin esterase of Lactococcus lactis was cloned using degenerate DNA probes based on 19 known N-terminal amino acid residues of the purified enzyme. The gene, named estA, was sequenced and found to encode a protein of 258 amino acid residues. The transc...

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Εκδόθηκε σε:	Appl Environ Microbiol
Κύριοι συγγραφείς:	Fernández, Leonides, Beerthuyzen, Marke M., Brown, Julie, Siezen, Roland J., Coolbear, Tim, Holland, Ross, Kuipers, Oscar P.
Μορφή:	Artigo
Γλώσσα:	Inglês
Έκδοση:	American Society for Microbiology (ASM) 2000
Θέματα:	Genetics and Molecular Biology
Διαθέσιμο Online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC91993/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10742212/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.66.4.1360-1368.2000
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