Simultaneous Enhancement of Thermostability and Catalytic Activity of Phospholipase A(1) by Evolutionary Molecular Engineering

The thermal stability and catalytic activity of phospholipase A(1) from Serratia sp. strain MK1 were improved by evolutionary molecular engineering. Two thermostable mutants were isolated after sequential rounds of error-prone PCR performed to introduce random mutations and filter-based screening of...

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Bibliografiske detaljer

Udgivet i:	Appl Environ Microbiol
Principais autores:	Song, Jae Kwang, Rhee, Joon Shick
Format:	Artigo
Sprog:	Inglês
Udgivet:	American Society for Microbiology (ASM) 2000
Fag:	Enzymology and Protein Engineering
Online adgang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC91918/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10698747/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.66.3.890-894.2000
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