Simultaneous Enhancement of Thermostability and Catalytic Activity of Phospholipase A(1) by Evolutionary Molecular Engineering

The thermal stability and catalytic activity of phospholipase A(1) from Serratia sp. strain MK1 were improved by evolutionary molecular engineering. Two thermostable mutants were isolated after sequential rounds of error-prone PCR performed to introduce random mutations and filter-based screening of...

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Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	Song, Jae Kwang, Rhee, Joon Shick
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 2000
Assuntos:	Enzymology and Protein Engineering
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC91918/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10698747/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.66.3.890-894.2000
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