Simultaneous Enhancement of Thermostability and Catalytic Activity of Phospholipase A(1) by Evolutionary Molecular Engineering
The thermal stability and catalytic activity of phospholipase A(1) from Serratia sp. strain MK1 were improved by evolutionary molecular engineering. Two thermostable mutants were isolated after sequential rounds of error-prone PCR performed to introduce random mutations and filter-based screening of...
Kaydedildi:
| Yayımlandı: | Appl Environ Microbiol |
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| Asıl Yazarlar: | , |
| Materyal Türü: | Artigo |
| Dil: | Inglês |
| Baskı/Yayın Bilgisi: |
American Society for Microbiology (ASM)
2000
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| Konular: | |
| Online Erişim: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC91918/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10698747/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.66.3.890-894.2000 |
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