A Sandwiched-Culture Technique for Evaluation of Heterologous Protein Production in a Filamentous Fungus

Aspergillus niger is known for its efficient excretion machinery. However, problems have often arisen in obtaining high amounts of heterologous proteins in the culture medium. Here we present a quick method using sandwiched colonies to evaluate transgenic strains for secretion of heterologous protei...

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Detalles Bibliográficos

Publicado en:	Appl Environ Microbiol
Principais autores:	Ásgeirsdóttir, Sigrídur A., Scholtmeijer, Karin, Wessels, Joseph G. H.
Formato:	Artigo
Idioma:	Inglês
Publicado:	American Society for Microbiology (ASM) 1999
Assuntos:	Physiology and Biotechnology
Acceso en liña:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC91327/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10224030/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.65.5.2250-2252.1999
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