A Sandwiched-Culture Technique for Evaluation of Heterologous Protein Production in a Filamentous Fungus
Aspergillus niger is known for its efficient excretion machinery. However, problems have often arisen in obtaining high amounts of heterologous proteins in the culture medium. Here we present a quick method using sandwiched colonies to evaluate transgenic strains for secretion of heterologous protei...
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| Veröffentlicht in: | Appl Environ Microbiol |
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| Hauptverfasser: | , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
American Society for Microbiology (ASM)
1999
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| Schlagworte: | |
| Online-Zugang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC91327/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10224030/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.65.5.2250-2252.1999 |
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