Isolation and Characterization of a Second Subunit of Molecular Chaperonin from Pyrococcus kodakaraensis KOD1: Analysis of an ATPase-Deficient Mutant Enzyme

The cpkA gene encoding a second (α) subunit of archaeal chaperonin from Pyrococcus kodakaraensis KOD1 was cloned, sequenced, and expressed in Escherichia coli. Recombinant CpkA was studied for chaperonin functions in comparison with CpkB (β subunit). The effect on decreasing the insoluble form of pr...

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Argitaratua izan da:	Appl Environ Microbiol
Egile Nagusiak:	Izumi, Michi, Fujiwara, Shinsuke, Takagi, Masahiro, Kanaya, Shigenori, Imanaka, Tadayuki
Formatua:	Artigo
Hizkuntza:	Inglês
Argitaratua:	American Society for Microbiology (ASM) 1999
Gaiak:	Enzymology and Protein Engineering
Sarrera elektronikoa:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC91257/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10103287/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.65.4.1801-1805.1999
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