Isolation and Characterization of a Second Subunit of Molecular Chaperonin from Pyrococcus kodakaraensis KOD1: Analysis of an ATPase-Deficient Mutant Enzyme

The cpkA gene encoding a second (α) subunit of archaeal chaperonin from Pyrococcus kodakaraensis KOD1 was cloned, sequenced, and expressed in Escherichia coli. Recombinant CpkA was studied for chaperonin functions in comparison with CpkB (β subunit). The effect on decreasing the insoluble form of pr...

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Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	Izumi, Michi, Fujiwara, Shinsuke, Takagi, Masahiro, Kanaya, Shigenori, Imanaka, Tadayuki
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1999
Assuntos:	Enzymology and Protein Engineering
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC91257/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10103287/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.65.4.1801-1805.1999
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