Isolation and Characterization of a Second Subunit of Molecular Chaperonin from Pyrococcus kodakaraensis KOD1: Analysis of an ATPase-Deficient Mutant Enzyme

The cpkA gene encoding a second (α) subunit of archaeal chaperonin from Pyrococcus kodakaraensis KOD1 was cloned, sequenced, and expressed in Escherichia coli. Recombinant CpkA was studied for chaperonin functions in comparison with CpkB (β subunit). The effect on decreasing the insoluble form of pr...

Täydet tiedot

Bibliografiset tiedot

Julkaisussa:	Appl Environ Microbiol
Päätekijät:	Izumi, Michi, Fujiwara, Shinsuke, Takagi, Masahiro, Kanaya, Shigenori, Imanaka, Tadayuki
Aineistotyyppi:	Artigo
Kieli:	Inglês
Julkaistu:	American Society for Microbiology (ASM) 1999
Aiheet:	Enzymology and Protein Engineering
Linkit:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC91257/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10103287/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.65.4.1801-1805.1999
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