Counting and Size Classification of Active Soil Bacteria by Fluorescence In Situ Hybridization with an rRNA Oligonucleotide Probe

A fluorescence in situ hybridization (FISH) technique based on binding of a rhodamine-labelled oligonucleotide probe to 16S rRNA was used to estimate the numbers of ribosome-rich bacteria in soil samples. Such bacteria, which have high cellular rRNA contents, were assumed to be active (and growing)...

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Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	Christensen, Henrik, Hansen, Michael, Sørensen, Jan
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1999
Assuntos:	General Microbial Ecology
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC91247/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10103277/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.65.4.1753-1761.1999
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