Modes of Action of Acarbose Hydrolysis and Transglycosylation Catalyzed by a Thermostable Maltogenic Amylase, the Gene for Which Was Cloned from a Thermus Strain

A maltogenic amylase gene was cloned in Escherichia coli from a gram-negative thermophilic bacterium, Thermus strain IM6501. The gene encoded an enzyme (ThMA) with a molecular mass of 68 kDa which was expressed by the expression vector p6xHis119. The optimal temperature of ThMA was 60°C, which was h...

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Veröffentlicht in:	Appl Environ Microbiol
Hauptverfasser:	Kim, Tae-Jip, Kim, Myo-Jeong, Kim, Byung-Cheon, Kim, Jae-Cherl, Cheong, Tae-Kyou, Kim, Jung-Wan, Park, Kwan-Hwa
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 1999
Schlagworte:	Enzymology and Protein Engineering
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC91232/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10103262/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.65.4.1644-1651.1999
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