New Mobilizable Vectors Suitable for Gene Replacement in Gram-Negative Bacteria and Their Use in Mapping of the 3′ End of the Xanthomonas campestris pv. campestris gum Operon

We describe useful vectors to select double-crossover events directly in site-directed marker exchange mutagenesis in gram-negative bacteria. These vectors contain the gusA marker gene, providing colorimetric screens to identify bacteria harboring those sequences. The applicability of these vectors...

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Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	Katzen, Federico, Becker, Anke, Ielmini, M. Verónica, Oddo, Cristian G., Ielpi, Luis
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1999
Assuntos:	Methods
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC91013/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9872790/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.65.1.278-282.1999
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