New Mobilizable Vectors Suitable for Gene Replacement in Gram-Negative Bacteria and Their Use in Mapping of the 3′ End of the Xanthomonas campestris pv. campestris gum Operon

We describe useful vectors to select double-crossover events directly in site-directed marker exchange mutagenesis in gram-negative bacteria. These vectors contain the gusA marker gene, providing colorimetric screens to identify bacteria harboring those sequences. The applicability of these vectors...

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Autors principals: Katzen, Federico, Becker, Anke, Ielmini, M. Verónica, Oddo, Cristian G., Ielpi, Luis
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology 1999
Matèries:
Methods
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC91013/
https://ncbi.nlm.nih.gov/pubmed/9872790
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