New Mobilizable Vectors Suitable for Gene Replacement in Gram-Negative Bacteria and Their Use in Mapping of the 3′ End of the Xanthomonas campestris pv. campestris gum Operon
We describe useful vectors to select double-crossover events directly in site-directed marker exchange mutagenesis in gram-negative bacteria. These vectors contain the gusA marker gene, providing colorimetric screens to identify bacteria harboring those sequences. The applicability of these vectors...
Uloženo v:
| Vydáno v: | Appl Environ Microbiol |
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| Hlavní autoři: | , , , , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
American Society for Microbiology (ASM)
1999
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC91013/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9872790/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.65.1.278-282.1999 |
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