Expression of Active Human Tissue-Type Plasminogen Activator in Escherichia coli

The formation of native disulfide bonds in complex eukaryotic proteins expressed in Escherichia coli is extremely inefficient. Tissue plasminogen activator (tPA) is a very important thrombolytic agent with 17 disulfides, and despite numerous attempts, its expression in an active form in bacteria has...

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Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	Qiu, Ji, Swartz, James R., Georgiou, George
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1998
Assuntos:	Enzymology and Protein Engineering
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC90939/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9835579/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.64.12.4891-4896.1998
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