Overexpression and Characterization of the Chromosomal Aminoglycoside 2′-N-Acetyltransferase of Providencia stuartii

The gene coding for aminoglycoside 2′-N-acetyltransferase Ia [AAC(2′)-Ia] from Providencia stuartii was amplified by PCR and cloned. The resulting construct, pACKF2, was transferred into Escherichia coli for overexpression of AAC(2′)-Ia as a fusion protein with an N-terminal hexa-His tag. The fusion...

Full beskrivning

Bibliografiska uppgifter

I publikationen:	Antimicrob Agents Chemother
Huvudupphov:	Franklin, Kristyn, Clarke, Anthony J.
Materialtyp:	Artigo
Språk:	Inglês
Utgiven:	American Society for Microbiology (ASM) 2001
Ämnen:	Mechanisms of Action: Physiological Effects
Länkar:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC90637/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11451680/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AAC.45.8.2238-2244.2001
Taggar:	Lägg till en tagg Inga taggar, Lägg till första taggen!