Overexpression and Characterization of the Chromosomal Aminoglycoside 2′-N-Acetyltransferase of Providencia stuartii

The gene coding for aminoglycoside 2′-N-acetyltransferase Ia [AAC(2′)-Ia] from Providencia stuartii was amplified by PCR and cloned. The resulting construct, pACKF2, was transferred into Escherichia coli for overexpression of AAC(2′)-Ia as a fusion protein with an N-terminal hexa-His tag. The fusion...

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Detalhes bibliográficos

Publicado no:	Antimicrob Agents Chemother
Principais autores:	Franklin, Kristyn, Clarke, Anthony J.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 2001
Assuntos:	Mechanisms of Action: Physiological Effects
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC90637/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11451680/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AAC.45.8.2238-2244.2001
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