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Overexpression and Characterization of the Chromosomal Aminoglycoside 2′-N-Acetyltransferase of Providencia stuartii

The gene coding for aminoglycoside 2′-N-acetyltransferase Ia [AAC(2′)-Ia] from Providencia stuartii was amplified by PCR and cloned. The resulting construct, pACKF2, was transferred into Escherichia coli for overexpression of AAC(2′)-Ia as a fusion protein with an N-terminal hexa-His tag. The fusion...

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Main Authors: Franklin, Kristyn, Clarke, Anthony J.
格式: Artigo
語言:Inglês
出版: American Society for Microbiology 2001
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC90637/
https://ncbi.nlm.nih.gov/pubmed/11451680
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AAC.45.8.2238-2244.2001
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