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Overexpression and Characterization of the Chromosomal Aminoglycoside 2′-N-Acetyltransferase of Providencia stuartii
The gene coding for aminoglycoside 2′-N-acetyltransferase Ia [AAC(2′)-Ia] from Providencia stuartii was amplified by PCR and cloned. The resulting construct, pACKF2, was transferred into Escherichia coli for overexpression of AAC(2′)-Ia as a fusion protein with an N-terminal hexa-His tag. The fusion...
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| Hoofdauteurs: | , |
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| Formaat: | Artigo |
| Taal: | Inglês |
| Gepubliceerd in: |
American Society for Microbiology
2001
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| Onderwerpen: | |
| Online toegang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC90637/ https://ncbi.nlm.nih.gov/pubmed/11451680 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AAC.45.8.2238-2244.2001 |
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