Biochemical Characterization of the FEZ-1 Metallo-β-Lactamase of Legionella gormanii ATCC 33297(T) Produced in Escherichia coli

The bla(FEZ-1) gene coding for the metallo-β-lactamase of Legionella (Fluoribacter) gormanii ATCC 33297(T) was overexpressed via a T7 expression system in Escherichia coli BL21(DE3)(pLysS). The product was purified to homogeneity in two steps with a yield of 53%. The FEZ-1 metallo-β-lactamase exhibi...

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Pubblicato in:	Antimicrob Agents Chemother
Autori principali:	Mercuri, Paola Sandra, Bouillenne, Fabrice, Boschi, Letizia, Lamotte-Brasseur, Josette, Amicosante, Gianfranco, Devreese, Bart, van Beeumen, Jozef, Frère, Jean-Marie, Rossolini, Gian Maria, Galleni, Moreno
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 2001
Soggetti:	Mechanisms of Resistance
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC90452/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11257043/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AAC.45.4.1254-1262.2001
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