Overproduction and Biochemical Characterization of the Chryseobacterium meningosepticum BlaB Metallo-β-Lactamase

The BlaB metallo-β-lactamase of Chryseobacterium meningosepticum CCUG4310 was overproduced in Escherichia coli by means of a T7 promoter-based expression system. The overproducing system, scaled up in a 15-liter fermentor, yielded approximately 10 mg of BlaB protein per liter, mostly released in the...

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Detalhes bibliográficos
Main Authors: Vessillier, Sandrine, Docquier, Jean-Denis, Rival, Sandrine, Frere, Jean-Marie, Galleni, Moreno, Amicosante, Gianfranco, Rossolini, Gian Maria, Franceschini, Nicola
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2002
Assuntos:
Mechanisms of Resistance
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC127231/
https://ncbi.nlm.nih.gov/pubmed/12019109
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AAC.46.6.1921-1927.2002
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