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Overproduction and Biochemical Characterization of the Chryseobacterium meningosepticum BlaB Metallo-β-Lactamase
The BlaB metallo-β-lactamase of Chryseobacterium meningosepticum CCUG4310 was overproduced in Escherichia coli by means of a T7 promoter-based expression system. The overproducing system, scaled up in a 15-liter fermentor, yielded approximately 10 mg of BlaB protein per liter, mostly released in the...
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| Main Authors: | , , , , , , , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
American Society for Microbiology
2002
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC127231/ https://ncbi.nlm.nih.gov/pubmed/12019109 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AAC.46.6.1921-1927.2002 |
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