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Biochemical Characterization of the FEZ-1 Metallo-β-Lactamase of Legionella gormanii ATCC 33297(T) Produced in Escherichia coli
The bla(FEZ-1) gene coding for the metallo-β-lactamase of Legionella (Fluoribacter) gormanii ATCC 33297(T) was overexpressed via a T7 expression system in Escherichia coli BL21(DE3)(pLysS). The product was purified to homogeneity in two steps with a yield of 53%. The FEZ-1 metallo-β-lactamase exhibi...
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| Main Authors: | , , , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
American Society for Microbiology
2001
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC90452/ https://ncbi.nlm.nih.gov/pubmed/11257043 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AAC.45.4.1254-1262.2001 |
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