Biochemical Characterization of the FEZ-1 Metallo-β-Lactamase of Legionella gormanii ATCC 33297(T) Produced in Escherichia coli

The bla(FEZ-1) gene coding for the metallo-β-lactamase of Legionella (Fluoribacter) gormanii ATCC 33297(T) was overexpressed via a T7 expression system in Escherichia coli BL21(DE3)(pLysS). The product was purified to homogeneity in two steps with a yield of 53%. The FEZ-1 metallo-β-lactamase exhibi...

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Bibliografische gegevens
Hoofdauteurs: Mercuri, Paola Sandra, Bouillenne, Fabrice, Boschi, Letizia, Lamotte-Brasseur, Josette, Amicosante, Gianfranco, Devreese, Bart, van Beeumen, Jozef, Frère, Jean-Marie, Rossolini, Gian Maria, Galleni, Moreno
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: American Society for Microbiology 2001
Onderwerpen:
Mechanisms of Resistance
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC90452/
https://ncbi.nlm.nih.gov/pubmed/11257043
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AAC.45.4.1254-1262.2001
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