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Use of Real-Time PCR and Fluorimetry To Detect Lamivudine Resistance-Associated Mutations in Hepatitis B Virus

Very rapid amplification of DNA by PCR in small volumes can be continuously monitored by the detection of the binding of probes with a rapid cycler with built-in fluorometric detection. Primers were designed to amplify approximately 100 bp of the polymerase gene of hepatitis B virus (HBV) spanning c...

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Main Authors: Cane, Patricia A., Cook, Pamela, Ratcliffe, Daina, Mutimer, David, Pillay, Deenan
Formato: Artigo
Idioma:Inglês
Publicado: American Society for Microbiology 1999
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC89331/
https://ncbi.nlm.nih.gov/pubmed/10390210
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