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Replicate PCR Testing and Probit Analysis for Detection and Quantitation of Chlamydia pneumoniae in Clinical Specimens

Nucleic acid amplification of clinical specimens with low target concentration has variable sensitivity. We examined whether testing multiple aliquots of extracted DNA increased the sensitivity and reproducibility of Chlamydia pneumoniae detection by PCR. Nested and non-nested C. pneumoniae PCR assa...

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Main Authors: Smieja, M., Mahony, J. B., Goldsmith, C. H., Chong, S., Petrich, A., Chernesky, M.
格式: Artigo
語言:Inglês
出版: American Society for Microbiology 2001
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC88028/
https://ncbi.nlm.nih.gov/pubmed/11325993
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.39.5.1796-1801.2001
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