Replicate PCR Testing and Probit Analysis for Detection and Quantitation of Chlamydia pneumoniae in Clinical Specimens

Nucleic acid amplification of clinical specimens with low target concentration has variable sensitivity. We examined whether testing multiple aliquots of extracted DNA increased the sensitivity and reproducibility of Chlamydia pneumoniae detection by PCR. Nested and non-nested C. pneumoniae PCR assa...

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Main Authors: Smieja, M., Mahony, J. B., Goldsmith, C. H., Chong, S., Petrich, A., Chernesky, M.
Formato: Artigo
Idioma:Inglês
Publicado: American Society for Microbiology 2001
Assuntos:
Chlamydiology and Rickettsiology
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC88028/
https://ncbi.nlm.nih.gov/pubmed/11325993
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.39.5.1796-1801.2001
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