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Replicate PCR Testing and Probit Analysis for Detection and Quantitation of Chlamydia pneumoniae in Clinical Specimens

Nucleic acid amplification of clinical specimens with low target concentration has variable sensitivity. We examined whether testing multiple aliquots of extracted DNA increased the sensitivity and reproducibility of Chlamydia pneumoniae detection by PCR. Nested and non-nested C. pneumoniae PCR assa...

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Udgivet i:J Clin Microbiol
Principais autores: Smieja, M., Mahony, J. B., Goldsmith, C. H., Chong, S., Petrich, A., Chernesky, M.
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 2001
Fag:
Chlamydiology and Rickettsiology
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC88028/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11325993/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JCM.39.5.1796-1801.2001
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