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Extraction and Genotyping of Cryptosporidium parvum DNA from Fecal Smears on Glass Slides Stained Conventionally for Direct Microscope Examination

A method was developed for extracting cryptosporidial DNA from stained fecal smears on glass microscope slides. The correct genotype of Cryptosporidium parvum was amplified by PCR from 89 (85%) of 105 smears following conventional staining but not from negative controls. This technique may have appl...

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Udgivet i:J Clin Microbiol
Principais autores: Amar, C., Pedraza-Díaz, S., McLauchlin, J.
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 2001
Fag:
Parasitology
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC87744/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11136813/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JCM.39.1.401-403.2001
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