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Extraction and Genotyping of Cryptosporidium parvum DNA from Fecal Smears on Glass Slides Stained Conventionally for Direct Microscope Examination
A method was developed for extracting cryptosporidial DNA from stained fecal smears on glass microscope slides. The correct genotype of Cryptosporidium parvum was amplified by PCR from 89 (85%) of 105 smears following conventional staining but not from negative controls. This technique may have appl...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology
2001
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC87744/ https://ncbi.nlm.nih.gov/pubmed/11136813 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.39.1.401-403.2001 |
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