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Extraction and Genotyping of Cryptosporidium parvum DNA from Fecal Smears on Glass Slides Stained Conventionally for Direct Microscope Examination

A method was developed for extracting cryptosporidial DNA from stained fecal smears on glass microscope slides. The correct genotype of Cryptosporidium parvum was amplified by PCR from 89 (85%) of 105 smears following conventional staining but not from negative controls. This technique may have appl...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Amar, C., Pedraza-Díaz, S., McLauchlin, J.
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology 2001
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC87744/
https://ncbi.nlm.nih.gov/pubmed/11136813
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.39.1.401-403.2001
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