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Rapid Detection of Enterohemorrhagic Escherichia coli by Real-Time PCR with Fluorescent Hybridization Probes
In this report, we present a PCR protocol for rapid identification of enterohemorrhagic Escherichia coli on a LightCycler instrument. In a multiplex assay, the genes encoding Shiga toxin 1 and Shiga toxin 2 are detected in a single reaction capillary. A complete analysis of up to 32 samples takes ab...
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| Main Authors: | , , , , |
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| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
American Society for Microbiology
2001
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC87735/ https://ncbi.nlm.nih.gov/pubmed/11136804 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.39.1.370-374.2001 |
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