Rapid Detection of Enterohemorrhagic Escherichia coli by Real-Time PCR with Fluorescent Hybridization Probes

In this report, we present a PCR protocol for rapid identification of enterohemorrhagic Escherichia coli on a LightCycler instrument. In a multiplex assay, the genes encoding Shiga toxin 1 and Shiga toxin 2 are detected in a single reaction capillary. A complete analysis of up to 32 samples takes ab...

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Detalhes bibliográficos
Main Authors: Bellin, Tobias, Pulz, Matthias, Matussek, Andreas, Hempen, Hans-Günther, Gunzer, Florian
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2001
Assuntos:
Bacteriology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC87735/
https://ncbi.nlm.nih.gov/pubmed/11136804
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.39.1.370-374.2001
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