Rapid Detection of Enterohemorrhagic Escherichia coli by Real-Time PCR with Fluorescent Hybridization Probes

In this report, we present a PCR protocol for rapid identification of enterohemorrhagic Escherichia coli on a LightCycler instrument. In a multiplex assay, the genes encoding Shiga toxin 1 and Shiga toxin 2 are detected in a single reaction capillary. A complete analysis of up to 32 samples takes ab...

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Detalhes bibliográficos

Publicado no:	J Clin Microbiol
Principais autores:	Bellin, Tobias, Pulz, Matthias, Matussek, Andreas, Hempen, Hans-Günther, Gunzer, Florian
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 2001
Assuntos:	Bacteriology
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC87735/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11136804/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JCM.39.1.370-374.2001
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