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Evaluation of 5′ Nuclease Assay for Detection of Actinobacillus pleuropneumoniae

Sequence detection by the 5′ nuclease TaqMan assay uses online detection of internal fluorogenic probes in closed PCR tubes. Primers and probe were chosen from a part of the omlA gene common to all serotypes of Actinobacillus pleuropneumoniae, which gave an amplicon of 92 bp. The test was evaluated...

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Bibliografski detalji
Glavni autori: Angen, Øystein, Jensen, Jannie, Lavritsen, Dorte T.
Format: Artigo
Jezik:Inglês
Izdano: American Society for Microbiology 2001
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC87711/
https://ncbi.nlm.nih.gov/pubmed/11136780
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.39.1.260-265.2001
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