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Evaluation of 5′ Nuclease Assay for Detection of Actinobacillus pleuropneumoniae
Sequence detection by the 5′ nuclease TaqMan assay uses online detection of internal fluorogenic probes in closed PCR tubes. Primers and probe were chosen from a part of the omlA gene common to all serotypes of Actinobacillus pleuropneumoniae, which gave an amplicon of 92 bp. The test was evaluated...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology
2001
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC87711/ https://ncbi.nlm.nih.gov/pubmed/11136780 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.39.1.260-265.2001 |
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