Molecular Cloning and Sequencing of the aroA Gene from Actinobacillus pleuropneumoniae and Its Use in a PCR Assay for Rapid Identification

The gene (aroA) of Actinobacillus pleuropneumoniae, serotype 2, encoding 5-enolpyruvylshikimate-3-phosphate synthase was cloned by complementation of the aroA mutation in Escherichia coli K-12 strain AB2829, and the nucleotide sequence was determined. A pair of primers from the 5′ and 3′ termini wer...

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Detalhes bibliográficos

Publicado no:	J Clin Microbiol
Principais autores:	Moral, Carmen Hernanz, Soriano, Alberto Cascón, Salazar, María Sánchez, Marcos, Javier Yugueros, Ramos, Susana Suárez, Carrasco, German Naharro
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1999
Assuntos:	Clinical Veterinary Microbiology
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC84834/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10203526/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.37.5.1575-1578.1999
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