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Molecular Cloning and Sequencing of the aroA Gene from Actinobacillus pleuropneumoniae and Its Use in a PCR Assay for Rapid Identification

The gene (aroA) of Actinobacillus pleuropneumoniae, serotype 2, encoding 5-enolpyruvylshikimate-3-phosphate synthase was cloned by complementation of the aroA mutation in Escherichia coli K-12 strain AB2829, and the nucleotide sequence was determined. A pair of primers from the 5′ and 3′ termini wer...

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Bibliografiska uppgifter
Huvudupphovsmän: Moral, Carmen Hernanz, Soriano, Alberto Cascón, Salazar, María Sánchez, Marcos, Javier Yugueros, Ramos, Susana Suárez, Carrasco, German Naharro
Materialtyp: Artigo
Språk:Inglês
Publicerad: American Society for Microbiology 1999
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Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC84834/
https://ncbi.nlm.nih.gov/pubmed/10203526
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