Detection of Legionella pneumophila Using a Real-Time PCR Hybridization Assay

A real-time PCR hybridization assay for Legionella pneumophila is described; the assay uses LightCycler (Idaho Technology) methodology to specifically detect 2.5 CFU/reaction, equivalent to 1,000 CFU/liter of starting water sample. The assay, including DNA extraction and confirmation of product iden...

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Detalles Bibliográficos
Main Authors: Ballard, A. L., Fry, N. K., Chan, L., Surman, S. B., Lee, J. V., Harrison, T. G., Towner, K. J.
Formato: Artigo
Idioma:Inglês
Publicado: American Society for Microbiology 2000
Assuntos:
Bacteriology
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC87566/
https://ncbi.nlm.nih.gov/pubmed/11060093
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