Detection of Legionella pneumophila Using a Real-Time PCR Hybridization Assay

A real-time PCR hybridization assay for Legionella pneumophila is described; the assay uses LightCycler (Idaho Technology) methodology to specifically detect 2.5 CFU/reaction, equivalent to 1,000 CFU/liter of starting water sample. The assay, including DNA extraction and confirmation of product iden...

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Pubblicato in:	J Clin Microbiol
Autori principali:	Ballard, A. L., Fry, N. K., Chan, L., Surman, S. B., Lee, J. V., Harrison, T. G., Towner, K. J.
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 2000
Soggetti:	Bacteriology
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC87566/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11060093/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.38.11.4215-4218.2000
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